Medchemexpress/Verteporfin(Synonyms: CL 318952)/HY-B0146/100mg,MCE,,MCE,MedChemExp,MedChemExpress,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Medchemexpress/Verteporfin(Synonyms: CL 318952)/HY-B0146/100mg

产品编号：HY-B0146-10mM*1mLinDMSO

市场价：¥3040.00

场地：美国(厂家直采)

产品分类：生化试剂>生物分子>小分子>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$152.00

品牌： MCE

公司：Medchemexpress

公司分类：

商品介绍

VerteporfinisabenzoporphyrinderivativemonoacidringA,andcaninhibittheactivityofYAP.

Description

VerteporfinisabenzoporphyrinderivativemonoacidringA,andcaninhibittheactivityofYAP.

InVitro

VerteporfinisspecificallyselectedbyPDX-cellscreening.Theconcentrationstocause50%growthinhibition(GI₅₀)forPhLO,PhLH,andPhLKare228nM,395nM,and538nM,respectively,whereasGI₅₀forALL-1,TCC-Y/sr,andNPhA1are3.93µM,2.11µM,and5.61µM,respectively.GSHsignificantlyreducesthesensitivityof2outof3PDXcellstoverteporfin.VerteporfinreducesthemitochondrialmembranepotentialinPDXcells^[1].VerteporfinreducesthePTX-resistanceonHCT-8/TcellsbyinhibitingYAPexpressionandcombinationtherapywithverteporfinandpaclitaxel(PTX)showssynergismoninhibitionofYAPandcytotoxicitytoHCT-8/T^[2].

InVivo

Verteporfin(10mg/kg,c.s.c.)anddasatinibsignificantlyreducestheleukemiacellratio,andcombinedtherapyfurtherreducedthenumberofleukemiacellsinthespleen^[1].

ClinicalTrial

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References

[1].MorishitaT,etal.Thephotosensitizerverteporfinhaslight-independentanti-leukemicactivityforPh-positiveacutelymphoblasticleukemiaandsynergisticallyworkswithdasatinib.Oncotarget.2016Aug2.
[2].PanW,etal.VerteporfincanReversethePaclitaxelResistanceInducedbyYAPOver-ExpressioninHCT-8/TCellswithoutPhotoactivationthroughInhibitingYAPExpression.CellPhysiolBiochem.2016;39(2):481-90.

PreparingStockSolutions

ConcentrationVolumeMass	1mg	5mg	10mg

1mM	1.3912mL	6.9561mL	13.9123mL
5mM	0.2782mL	1.3912mL	2.7825mL
10mM	0.1391mL	0.6956mL	1.3912mL

Pleaserefertothesolubilityinformationtoselecttheappropriatesolvent.

CellAssay
^[1]

VerteporfinisdissolvedinDMSO.

PDXcellsco-culturedwithS17cellsaretreatedwith16combinationsofverteporfin(60nM,120nM,180nM,and240nM)anddasatinib(12nM,24nM,36nM,and48nM).TheviABIlitiesofcellstreatedwitheachcombinationaremeasuredafter48husingFACSAriaflowcytometer.Inordertoestimatedruginteractionbetweenverteporfinanddasatinib,anormalizedisobologramandfractionaffectedcombinationindex(CI)plotaremadeusingCompuSynsoftware.CIvaluesgreaterthan1.0indicatedantagoNISTiceffects,equalto1.0additiveeffects,andbelow1.0synergisticeffects.MCEhasnotindependentlyconfirmedtheaccuracyofthesemethods.Theyareforreferenceonly.

AnimalAdministration
^[1]

PhLOcells(1.0×10⁷/mouse)areinjectedintravenouslyinto6-week-oldmaleNOGmice,whicharethentreatedwithvehicle,verteporfin(140mg/kg/day),dasatinib(20mg/kg/day),andacombinationofthesedrugsfromdays22to28.Verteporfinisadministeredbycontinuoussubcutaneousinfusion(c.s.c.)usingAlzetosmoticpumps.Anintraperitonealinjection(i.p.)isperformedfordasatinib.Allmicearesacrificedonday28andthechimerismofleukemiacellsisinvestigatedbyflowcytometerusingananti-humanCD19antibodyandantimouseCD45antibody.BloodconcentrationsofverteporfinarecalculatedbyLCMS-2020.MCEhasnotindependentlyconfirmedtheaccuracyofthesemethods.Theyareforreferenceonly.

References

[1].MorishitaT,etal.Thephotosensitizerverteporfinhaslight-independentanti-leukemicactivityforPh-positiveacutelymphoblasticleukemiaandsynergisticallyworkswithdasatinib.Oncotarget.2016Aug2.
[2].PanW,etal.VerteporfincanReversethePaclitaxelResistanceInducedbyYAPOver-ExpressioninHCT-8/TCellswithoutPhotoactivationthroughInhibitingYAPExpression.CellPhysiolBiochem.2016;39(2):481-90.

MolecularWeight

718.79

Formula

C₄₁H₄₂N₄O₈

CASNo.

129497-78-5

Storage

Powder	-20°C	3years
	4°C	2years
Insolvent	-80°C	6months
	-20°C	1month

Shipping

RoomtemperatureincontinentalUS;mayvaryelsewhere

Solvent&Solubility

DMSO:5.6mg/mL

*"<1 mg/ml"="" means="" slightly="" soluble="" or="" insoluble.="" "≥"="" means="" soluble,="" but="" saturation="">

Purity:99.26%

SelectBatch: